Departments

 

Molecular Sciences

In the Molecular Sciences Department, we have a fully equipped laboratory for performing Real-Time PCR, TaqMan Genotyping for Drug Metabolism Enzymes, DNA sequencing for Human Identification and Flow Cytometry for immunophenotyping. Our equipment include standard PCR machines, Applied Biosystems 7500 System, 3500 Sequencer, Partec Flow Cytometer, Veriti PCR System.


For more information please contact Dr. Roslyn Thelingwani on tel +263 4 740433 or e-mail This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it..

 

 

 

Dr. Roslyn Thelingwani

Medical Bioanalysis

The department has 6 members comprising of 2 PhD holders, 1 PhD and 3 Masters candidates with expertise in drug metabolism and analytical chemistry. Our main focus is bioanalysis and CYP mediate drug metabolism research. The department has state of the art equipment, which include high liquid pressure liquid chromatography machines coupled to mass and UV detectors. Currently we have 5 mass spectrometers and 2 diode array UV detectors. In addition we have spectrophotometers, multilabel plate readers and liquid handling systems which allow high throughput screening. We have also acquired FDA and industry recognised software such as NONMEM, SIMCA, Winonlin, SymCYP and the molecular Discovery Suite.

For more information please contact Dr. Roslyn Thelingwani on tel +263 4 7404733 or e-mail This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it..

 

 

Units

 

Medical Molecular Diagnostics

The Medical Molecular Diagnostics Unit performs diagnostic tests for monitoring HIV/AIDS patients who are on antiretroviral therapy for drug efficacy and safety including drug resistance. Diagnostic tests performed include immunophenotyping (CD4 and CD4% counts), HIV viral load determination and Hepatitis screening. Determination of drug resistance and subtypes is mainly done for HIV, TB and malaria. Our pharmacogenetics work focuses on genes affecting drug response and susceptibility to diseases e.g. Breast Cancer.

 

Forensic Sciences

The unit comprises of 7 members with expertise in the areas of bioanalysis, molecular biology and chemistry. The focus is on human identification and detection of counterfeit drugs. In human identification we are currently offering paternity/maternity testing and disaster victim identification. To achieve this the unit has acquired equipment, which include the 3500 Genetic analyser, 7500 Real Time PCR machine and the Veriti thermal cycler which are validated for human identification. In the analytical section we have mass spectrometers, HPLCs, robotic liquid handling systems, spectrophotometers and plate readers. We have also acquired the minilab for rapid detection of counterfeit drugs especially when in the field. The biggest project the unit has undertaken to date is the identification of 17 bodies burnt beyond recognition in a traffic accident that resulted in a fire. The accident occurred in Chisumbanje in Manicalnad Province of Zimbabwe.

 

ADMET/PK

Our main area of focus is Cytochrome P450 (CYP) mediated drug metabolism studies both in vitro and in vivo. We study the interaction of xenobiotics (drugs, herbs, food supplements, carcinogens) with CYP enzymes in an effort to have safer medicines. Over the years we have set up various in vitro and in vivo methods, and in silico tools. This has enabled the unit to do contract research work for various companies e.g. AstraZeneca and Merk, organisations such as (NIPRD) World Health Organisation - Tropical Disease Research (WHO-TDR) and Universities including University of Cape Town, University of North West and University of Zimbabwe. The unit has hosted various researchers for training from Nigeria, Kenya, South Africa and Sweden for training and collaboration.